胆汁酸对黄曲霉毒素B1生物蓄积、解毒系统的影响

摘要  

本研究评估了胆汁酸（BAs）太平洋白虾体内黄曲霉毒素B1（AFB1）残留及毒性的抑制作用。实验采用0、0.05、0.15和0.25 g/kg剂量的胆汁酸处理幼虾和亚成体60天，随后进行为期10天的AFB1暴露（2000μg/kg）。每个处理组选取15只虾（每组5只合并为一个样本，n = 3)，分别在30、60、63、66和70天五个时间点取样。所有参数均采用标准方法测定，准确度（回收率）达90%-110%。

结果显示：胆汁酸显著降低了虾体内AFB1残留量（检测限：0.01μg/L，相对标准偏差＜10%，回收率＞90%）。胆汁酸通过增强II相代谢系统和抗氧化机制，有效提升了AFB1的解毒能力，同时降低了氧化应激产物水平，从而避免了AFB1引发的肉质劣化及对人类健康的风险。实验置信水平为95%。。

亮点 

•胆汁酸可降低对虾体内AFB1的残留量。

•胆汁酸能增强对虾的解毒和抗氧化能力。

•胆汁酸可缓解AFB1对虾肉品质的不良影响。

•胆汁酸可降低人类接触AFB1的风险。

Abstract

The potential of bile acids (BAs) to reduce aflatoxin B1 (AFB1) residues and toxicity in Litopenaeus vannamei was evaluated. Both juveniles and subadults were treated with 0, 0.05, 0.15 and 0.25 g/kg BAs for 60 days followed by 10-d AFB1 exposure (2000 μg/kg), and fifteen shrimp (five shrimp were pooled into one sample, n = 3) from each treatment were collected at five time points (30, 60, 63, 66 and 70 d). All parameters were determined using accepted and standard methods with acceptable accuracy (recovery) of 90–110%. Results demonstrated that BAs reduced the AFB1 residues in shrimp (limit of detection: 0.01 μg/L, relative standard deviation < 10% and recovery: 92.1–96.8%). BAs increased the detoxification of AFB1 and decreased the levels of oxidative stress products by increasing Phase II and antioxidant systems, avoiding AFB1-induced deterioration of shrimp meat and health risks to human. The confidence level was 95%.

Highlights

•Bile acids reduced the AFB1 residues of Litopenaeus vannamei.

•Bile acids improved the detoxification and antioxidant systems of L. vannamei.

•Bile acid alleviated the deterioration of shrimp meat quality induced by AFB1.

•Bile acids reduced the risk of human exposure to AFB1.

编译：伏晓晓

原文：Chen S ,Yusong L ,Jinbao L , et al.Effects of bile acids on aflatoxin B1 bioaccumulation, detoxification system, and growth performance of Pacific white shrimp.[J].Food chemistry,2021,371131169-131169.

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