脂肪营养：雌虾的最佳脂肪添加量

亮点

11.01%脂肪水平显著促进了凡纳滨对虾卵巢发育阶段IV和V的百分比。

11.01%脂肪水平显著提高了血淋巴和卵巢中ECR和RXR的基因表达。

10.14%-14.65%的脂肪水平显著上调卵巢发育相关蛋白的表达（Vtg和Hsd3b1）。 

摘要

本研究旨在评估饲料中脂肪水平对凡纳滨对虾卵巢发育的影响。实验设置五个脂肪水平：8.87%、10.14%、11.01%、12.88%和14.65%，实验为期14周。结果表明，11.01%脂肪组卵巢中n-3长链多不饱和脂肪酸含量在所有处理中最高。11.01%脂肪组虾的卵巢发育至IV期和V期的比例最高。该组血淋巴中的甘油三酯、总胆固醇、雌二醇、5-羟色胺、卵黄蛋白原以及卵巢中卵黄蛋白原含量均显著高于其他处理组。11.01%脂肪饲料显著上调了卵巢发育相关基因(pcna、sesn、vg1、vgr和vtg)的表达，而大于10.14%脂肪组则显著提高了卵巢发育相关蛋白(Vtg/α-Tubulin和Hsd3b1/α-Tubulin)的表达水平。11.01%脂肪组对虾的脑部和卵巢中蜕皮相关基因(sad、rxr、e75、hr3和hr4)的表达量最高。饲料脂肪水平显著影响脂代谢相关基因的表达。综上所述，11.01%脂肪饲料有助于肝胰腺中外源性卵黄蛋白原的合成，并通过卵黄蛋白原受体将其转运至卵巢，进而促进卵巢内源性卵黄蛋白原的生成。

Highlights

•Dietary 11.01 % lipid significantly promoted percentage of IV and V ovarian developmental stages of L. vannamei.

•Dietary 11.01 % lipid significantly enhanced concentration of ECR and RXR in hemolymph and ovary.

•The protein expression of Vtg and Hsd3b1 significantly up-regulated by dietary 10.14 % ~ 14.65 % lipid.

Abstract

The purpose of this research was to evaluate the effect of dietary lipid levels on ovarian development for Pacific white shrimp(Litopenaeus vannamei). Five levels of lipid were prepared to contain 8.87 %, 10.14 %, 11.01 %, 12.88 % and 14.65 %, respectively. The feeding expreiment lasted for fourteen weeks. The results evidenced that 11.01 % lipid diet group showed the maximum n-3 LC-PUFA in ovary among all treatments. The highest proportion of ovarian maturation to stage IV and stage V occurred at the treatment with 11.01 % lipid. The treatment with 11.01 % lipid showed the highest TG, TCHO, E2, 5-HT, VTG in hemolymph, and VTG in ovary among all treatments. Dietary 11.01 % lipid notably up-regulated the expression of genes relevant to ovary development such as pcna, sesn, vg1, vgr and vtg in ovary, dietary excessive 10.14 % lipid can notably up-regulated the expression of protein related to ovary development such as Vtg / α-Tubulin and Hsd3b1 / α-Tubulin. The highest expression of brain and ovarian genes related to molting such as sad, rxr, e75, hr3 and hr4 existed at the treatment with 11.01 % lipid. Dietary lipid significantly affected lipid metabolism-related gene expression. In conclusion, dietary 11.01 % lipid contributed to the synthesis of hepatopancreatic exogenous vitellinogen, and transportation to the ovary via the vitellogenin receptor, further promoting the production of ovarian endogenous vitellinogen.

翻译:伏晓晓

原文：Lei T, Li M, Wang J, et al. Dietary lipid regulates ovarian development, molting and lipid metabolism via the RXR/ECR pathway in female Litopenaeus vannamei[J]. Aquaculture, 2025: 743086.

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